Physics Colloquium - Fall 2005 - "Quantitative Analysis of Energy Metabolism using Cellular NAD(P)H"
Dept of Physics & Astronomy
University of Maine, Orono, Maine
Presents
Ahmed A. Heikal, Ph.D.
Associate Professor
Department of Bioengineering
The Huck Institutes of the Life Sciences
The Pennsylvania State University
"Quantitative Analysis of Energy Metabolism using Cellular NAD(P)H"
Abstract: "The concentration of intrinsic biomolecules, such as proteins and cofactors, correlates with cellular processes and, therefore, can be used as a reporter for health and disease diagnoses. While biochemical techniques require lysing the cell, two-photon (2P) fluorescence dynamics is a non-invasive approach for functional imaging of these biomolecules in intact living cells. Recently, intrinsic cofactors, such as reduced pyridine nucleotides (NAD(P)H), are being at the center of research interest as a natural biosensor for mitochondrial metabolic activities in live cells and tissues. Furthermore, NAD(P)H can also be used as a diagnostic tool for health-related problems associated with mitochondrial anomalies. I will present our recent results on using ultrafast molecular dynamics and two-photon fluorescence microscopy of cellular autofluorescence, both in breast cancer cells and brain slices as model systems."
Friday, Nov. 11, 2005
3:10 pm
140 Bennett Hall
Refreshments will follow in Rm. 114, Bennett Hall
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